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Literature summary for 3.4.24.B3 extracted from

  • Holtz, B.; Cuniasse, P.; Boulay, A.; Kannan, R.; Mucha, A.; Beau, F.; Basset, P.; Dive, V.
    Role of the S1' subsite glutamine 215 in activity and specificity of stromelysin-3 by site-directed mutagenesis (1999), Biochemistry, 38, 12174-12179.
    View publication on PubMed
Search for more literature for 3.4.24.B3

Protein Variants

Protein Variants Comment Organism
Q215L site-directed mutagenesis, exchange of the residue preceeding the catalytic zinc-binding site results in altered substrate specificity for the P1' position of substrates compared to the wild-type Mus musculus
Q215R site-directed mutagenesis, exchange of the residue preceeding the catalytic zinc-binding site results in altered substrate specificity for the P1' position of substrates compared to the wild-type Mus musculus
Q215Y site-directed mutagenesis, exchange of the residue preceeding the catalytic zinc-binding site results in altered substrate specificity for the P1' position of substrates compared to the wild-type Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 dansyl-Pro-Leu-Ala-Leu-Trp-Ala-Arg-NH2 pH 6.8, 25°C, mutant Q215R Mus musculus
0.0045
-
 dansyl-Pro-Leu-Ala-S-[(4-methoxyphenyl)methyl]Cys-Trp-Ala-Arg-NH2 pH 6.8, 25°C, mutant Q215L Mus musculus
0.0052
-
 dansyl-Pro-Leu-Ala-S-[(4-methoxyphenyl)methyl]Cys-Trp-Ala-Arg-NH2 pH 6.8, 25°C, wild-type enzyme Mus musculus
0.0057
-
 dansyl-Pro-Leu-Ala-Leu-Trp-Ala-Arg-NH2 pH 6.8, 25°C, mutant Q215Y Mus musculus
0.0064
-
 dansyl-Pro-Leu-Ala-S-[(4-methoxyphenyl)methyl]Cys-Trp-Ala-Arg-NH2 pH 6.8, 25°C, mutant Q215R Mus musculus
0.021
-
 dansyl-Pro-Leu-Ala-Leu-Trp-Ala-Arg-NH2 pH 6.8, 25°C, wild-type enzyme Mus musculus
0.088
-
 dansyl-Pro-Leu-Ala-Leu-Trp-Ala-Arg-NH2 pH 6.8, 25°C, mutant Q215L Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Mus musculus
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity of wild-type and mutant enzymes Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha1-protease inhibitor + H2O wild-type enzyme and mutants Q215L, Q215R and Q215Y Mus musculus ?
-
 ?
dansyl-Pro-Leu-Ala-Leu-Trp-Ala-Arg-NH2 + H2O wild-type enzyme and mutants Q215L, Q215R and Q215Y Mus musculus ?
-
 ?
dansyl-Pro-Leu-Ala-S-p-methoxybenzylcysteine-Trp-Ala-Arg-NH2 + H2O wild-type and mutants Q215R and Q215L, mutant Q215Y shows no activity with this substrate Mus musculus ?
-
 ?
dansyl-Pro-Leu-Ala-S-[(4-methoxyphenyl)methyl]Cys-Trp-Ala-Arg-NH2 + H2O
-
 Mus musculus dansyl-Pro-Leu-Ala + S-[(4-methoxyphenyl)methyl]Cys-Trp-Ala-Arg-NH2
-
 ?
protein + H2O
-
 Mus musculus peptides
-
 ?

Synonyms

Synonyms Comment Organism
M10.007 Merops-ID Mus musculus
matrix metalloproteinase-11
-
 Mus musculus
MMP-11
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
 dansyl-Pro-Leu-Ala-Leu-Trp-Ala-Arg-NH2 pH 6.8, 25°C, wild-type enzyme Mus musculus
0.013
-
 dansyl-Pro-Leu-Ala-Leu-Trp-Ala-Arg-NH2 pH 6.8, 25°C, mutant Q215L Mus musculus
0.016
-
 dansyl-Pro-Leu-Ala-Leu-Trp-Ala-Arg-NH2 pH 6.8, 25°C, mutant Q215R Mus musculus
0.038
-
 dansyl-Pro-Leu-Ala-Leu-Trp-Ala-Arg-NH2 pH 6.8, 25°C, mutant Q215Y Mus musculus
0.078
-
 dansyl-Pro-Leu-Ala-S-[(4-methoxyphenyl)methyl]Cys-Trp-Ala-Arg-NH2 pH 6.8, 25°C, wild-type enzyme Mus musculus
0.083
-
 dansyl-Pro-Leu-Ala-S-[(4-methoxyphenyl)methyl]Cys-Trp-Ala-Arg-NH2 pH 6.8, 25°C, mutant Q215L Mus musculus
0.65
-
 dansyl-Pro-Leu-Ala-S-[(4-methoxyphenyl)methyl]Cys-Trp-Ala-Arg-NH2 pH 6.8, 25°C, mutant Q215R Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Mus musculus